Blakey D H, Bayley J M, Huang K C
Bureau of Chemical Hazards, Department of National Health and Welfare, Ottawa, Canada.
Mutagenesis. 1993 May;8(3):189-92. doi: 10.1093/mutage/8.3.189.
The development and use of chromosome-specific DNA probes to label entire human chromosomes has been an important advance in molecular cytogenetics. Fluorescence in situ hybridization using whole chromosome-specific DNA probes has been used to study both numerical and structural chromosomal aberrations in many human cell types. It would be useful if this technology could be applied to other animal species. However, whole chromosome-specific DNA probes have been reported for only human chromosomes. In this study, experiments were conducted to determine whether human probes could be used to label chromosomes of the cynomolgus monkey, Macaca fascicularis. The results demonstrate that some human DNA probes are suitable for the study of chromosomal aberrations in the monkey. Monkey chromosomes 1 and 4 labelled with human DNA probes had a strong, chromosome-specific labelling pattern. The probe for human chromosome 21 labelled the short arm on the monkey chromosome 2 and the probe for human chromosome 2 could not be detected on any of the monkey chromosomes.
染色体特异性DNA探针的开发与应用，用于标记整个人类染色体，这是分子细胞遗传学领域的一项重要进展。使用全染色体特异性DNA探针进行荧光原位杂交，已被用于研究多种人类细胞类型中的染色体数目和结构异常。如果这项技术能够应用于其他动物物种，那将是很有用的。然而，据报道，全染色体特异性DNA探针仅用于人类染色体。在本研究中，进行了实验以确定人类探针是否可用于标记食蟹猴（猕猴）的染色体。结果表明，一些人类DNA探针适用于研究猴子的染色体异常。用人类DNA探针标记的猴子1号和4号染色体呈现出强烈的、染色体特异性的标记模式。人类21号染色体的探针标记了猴子2号染色体的短臂，而人类2号染色体的探针在任何猴子染色体上均未检测到。
