Sen C K, Rahkila P, Hänninen O
Department of Physiology, University of Kuopio, Finland.
Acta Physiol Scand. 1993 May;148(1):21-6. doi: 10.1111/j.1748-1716.1993.tb09527.x.
Skeletal muscle derived L6 myoblasts possess a considerably high resting total glutathione (TGSH) pool. Exposure to L-buthionine-[S,R]-sulphoximine resulted in a 90% depletion of the intracellular TGSH pool. All the key enzymes of glutathione metabolism, especially glutathione S-transferase, were observed to be considerably active in the undifferentiated cells. Se-dependent glutathione peroxidase activity appeared to account for most of the total GSH peroxidase activity of the cells. A significant contribution of gamma-glutamyl transpeptidase-independent (5 mM acivicin insensitive) mechanism to the extracellular GSH uptake capacity of the muscle cells was evident. Efflux of oxidized glutathione from the cells exposed to t-butyl hydroperoxide was rapid and appeared to be energy linked.
骨骼肌来源的L6成肌细胞拥有相当高的静息总谷胱甘肽（TGSH）储备。暴露于L-丁硫氨酸-[S,R]-亚砜亚胺会导致细胞内TGSH储备减少90%。在未分化细胞中观察到谷胱甘肽代谢的所有关键酶，尤其是谷胱甘肽S-转移酶，具有相当高的活性。硒依赖性谷胱甘肽过氧化物酶活性似乎占细胞总谷胱甘肽过氧化物酶活性的大部分。γ-谷氨酰转肽酶非依赖性（5 mM阿西维辛不敏感）机制对肌肉细胞的细胞外谷胱甘肽摄取能力有显著贡献。暴露于叔丁基过氧化氢的细胞中氧化型谷胱甘肽的外流很快，并且似乎与能量有关。
