Sugimoto M, Imamura F, Matsumoto M, Sonoda E, Cho I, Ando M
First Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
Am J Trop Med Hyg. 1993 Jun;48(6):803-11. doi: 10.4269/ajtmh.1993.48.803.
It has been shown that T lymphocyte alveolitis occurs in patients with human T lymphotropic virus type-1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP). To determine whether HTLV-1-specific antibodies were present in bronchoalveolar lavage (BAL) fluid, we investigated 15 patients with HAM/TSP, five HTLV-1 carriers without HAM/TSP, and 10 normal control subjects seronegative for HTLV-1. Studies of BAL showed that 12 of 15 patients with HAM/TSP were associated with bronchoalveolar T lymphocytosis. Such abnormalities of the lung were not found in three other patients with HAM/TSP, non-HAM/TSP carriers, and normal control subjects. Using Western blots, specific IgA antibodies were detected only in BAL fluid from 12 patients with HAM/TSP and with pulmonary involvement. On the other hand, specific IgG antibodies in BAL fluid were positive not only in 12 patients with HAM/TSP and with pulmonary involvement, but also in two of three patients with HAM/TSP and three of five non-HAM/TSP carriers, both of whom showed normal BAL findings. Specific IgM class antibodies in BAL fluid were not detected in any subjects in these three groups. Specific IgA antibodies were found in the sera of 12 patients with HAM/TSP and with BAL lymphocytosis, but not in three patients with HAM/TSP and without BAL lymphocytosis, and five non-HAM/TSP carriers. These results suggest that the production of HTLV-1-specific IgA antibodies is closely related to pulmonary involvement in patients with HAM/TSP.
已表明，人类嗜T淋巴细胞病毒1型（HTLV-1）相关脊髓病/热带痉挛性截瘫（HAM/TSP）患者会发生T淋巴细胞肺泡炎。为确定支气管肺泡灌洗（BAL）液中是否存在HTLV-1特异性抗体，我们调查了15例HAM/TSP患者、5例无HAM/TSP的HTLV-1携带者以及10例HTLV-1血清学阴性的正常对照者。BAL研究显示，15例HAM/TSP患者中有12例伴有支气管肺泡T淋巴细胞增多。在其他3例HAM/TSP患者、非HAM/TSP携带者及正常对照者中未发现此类肺部异常。使用蛋白质印迹法，仅在12例有肺部受累的HAM/TSP患者的BAL液中检测到特异性IgA抗体。另一方面，BAL液中的特异性IgG抗体不仅在12例有肺部受累的HAM/TSP患者中呈阳性，在3例HAM/TSP患者中的2例以及5例非HAM/TSP携带者中的3例也呈阳性，这两组患者的BAL检查结果均正常。在这三组的任何受试者中均未在BAL液中检测到特异性IgM类抗体。在12例有BAL淋巴细胞增多的HAM/TSP患者的血清中发现了特异性IgA抗体，但在3例无BAL淋巴细胞增多的HAM/TSP患者及5例非HAM/TSP携带者中未发现。这些结果表明，HTLV-1特异性IgA抗体的产生与HAM/TSP患者的肺部受累密切相关。
