Lanzaro G C, Ostrovska K, Herrero M V, Lawyer P G, Warburg A
Department of Entomology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, District of Columbia.
Am J Trop Med Hyg. 1993 Jun;48(6):839-47. doi: 10.4269/ajtmh.1993.48.839.
The sand fly Lutzomyia longipalpis is the vector of Leishmania donovani chagasi in Latin America. An analysis of genetic variability at 27 enzyme coding loci among three laboratory populations of Lu. longipalpis revealed substantial genetic polymorphism. Levels of genetic distance between all pairwise comparisons of colonies were very high, and consistent with those previously reported among separate species in the genus Lutzomyia. Between 7% and 22% of the loci studied were diagnostic for any two of the colony populations. Experimental hybridization between colonies resulted in the production of sexually sterile male progeny. Our results provide strong evidence that Lu. longipalpis exists in nature as a complex of at least three distinct species. The possible effects of colonization on the genetic makeup of laboratory populations is considered in extending our results to natural populations.
沙蝇长须罗蛉是拉丁美洲杜氏利什曼原虫恰加斯亚种的传播媒介。对三个长须罗蛉实验室种群中27个酶编码位点的遗传变异性分析显示出大量的遗传多态性。所有菌落两两比较之间的遗传距离水平非常高，与之前在罗蛉属不同物种间报道的一致。所研究位点的7%至22%在任何两个菌落种群之间具有诊断性。菌落间的实验杂交产生了性不育的雄性后代。我们的结果提供了有力证据，表明长须罗蛉在自然界中以至少三个不同物种的复合体形式存在。在将我们的结果推广到自然种群时，考虑了定殖对实验室种群遗传组成的可能影响。
