商陆抗病毒蛋白和夫西地酸对体外蚕豆翻译的信使依赖性作用。

Messenger-dependent action of the pokeweed antiviral protein and fusidic acid on in vitro Vicia sativa L. translation.

作者信息

Arias F J, Rojo M A, Muñoz R, Iglesias R, Ferreras J M, Girbés T

机构信息

Departamento de Bioquímica, Biología Molecular y Fisiología, Facultad de Ciencias, Universidad de Valladolid, Spain.

出版信息

Cell Mol Biol (Noisy-le-grand). 1993 May;39(3):333-7.

PMID:8334385

Abstract

Plant ribosome sensitivity to either pokeweed antiviral protein (PAP) or fusidic acid has been investigated using a highly coupled translation system obtained from Vicia sativa L., a plant that lacks ribosome-inactivating protein activity. Protein synthesis directed by endogenous messengers was more sensitive to PAP than synthesis of polyphenylalanine directed by polyuridylic acid. Conversely, the effects of fusidic acid were the highest in polyphenylalanine synthesis. Additionally, the concentration of magnesium ions during translation also played a determinant role in the extent of inhibition by both PAP and fusidic acid. The effects seem to be exerted on the interaction of the translational elongation factor 2 with the ribosome.

摘要

利用从缺乏核糖体失活蛋白活性的植物蚕豆（Vicia sativa L.）中获得的高度偶联翻译系统，研究了植物核糖体对商陆抗病毒蛋白（PAP）或夫西地酸的敏感性。内源性信使指导的蛋白质合成比聚尿苷酸指导的聚苯丙氨酸合成对PAP更敏感。相反，夫西地酸对聚苯丙氨酸合成的影响最大。此外，翻译过程中镁离子的浓度在PAP和夫西地酸的抑制程度方面也起决定性作用。这些作用似乎是通过翻译延伸因子2与核糖体的相互作用来发挥的。