Lombardi V, Caniglia M, Scarlatti G, Jansson M, Plebani A, D'Argenio P, Scaccia S, Wigzell H, Rossi P
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Eur J Pediatr. 1993 Jun;152(6):484-9. doi: 10.1007/BF01955055.
The presence of specific IgA antibodies in sera from 25 infants born to HIV-1 seropositive mothers was investigated by peptide-ELISA and peptide time-resolved fluoro-immunoassay (TR-FIA). The infants had been monitored at different times after birth for clinical signs and/or symptoms of HIV-1 infection and for detection of HIV-1 in lymphocyte cultures. Serum samples had also been tested for HIV-1 IgG antibodies by commercial ELISA and Western blot and for p24 antigen. Eleven of 25 children were then identified as infected. IgA detection was performed after rProtein G treatment to remove interfering IgG. In the infected group, IgA specific antibodies to a synthetic peptide representing a highly conserved region of the transmembrane glycoprotein gp41 (env: 594-613) were detected in 27 (73%) out of 37 serum samples (9 of 11 children) by the peptide-ELISA test. IgA specific antibodies to the same peptide were found in 30 (81%) sera (9 of 11 children) by the peptide-TR-FIA. Specific HIV-1 IgA antibodies were detected as early as 2 months of age in serum samples from five out of seven children (71% sensitivity) using peptide-ELISA and from six out of seven (86% sensitivity) by peptide-TR-FIA. Conversely, IgA specific antibodies to HIV-1 were absent in two infected children as well as in the sera of all uninfected children tested during the follow up period. Since maternal IgA does not cross the placenta, IgA detection in the serum of the infant is indicative of HIV-1 infection.(ABSTRACT TRUNCATED AT 250 WORDS)
采用肽酶联免疫吸附测定法（ELISA）和肽时间分辨荧光免疫测定法（TR-FIA），对25名HIV-1血清反应阳性母亲所生婴儿血清中特异性IgA抗体的存在情况进行了研究。这些婴儿在出生后的不同时间接受了监测，以观察HIV-1感染的临床体征和/或症状，并检测淋巴细胞培养物中的HIV-1。血清样本还通过商业ELISA和蛋白质印迹法检测了HIV-1 IgG抗体以及p24抗原。随后，25名儿童中有11名被确定感染。在经rProtein G处理以去除干扰性IgG后进行IgA检测。在感染组中，通过肽ELISA试验，在37份血清样本中的27份（73%）（11名儿童中的9名）中检测到针对代表跨膜糖蛋白gp41高度保守区域（env: 594-613）的合成肽的IgA特异性抗体。通过肽TR-FIA在30份血清（81%）（11名儿童中的9名）中发现了针对同一肽的IgA特异性抗体。使用肽ELISA，在7名儿童中的5名（71%敏感性）血清样本中，早在2个月大时就检测到了特异性HIV-1 IgA抗体；通过肽TR-FIA，在7名儿童中的6名（86%敏感性）中检测到。相反，在两名感染儿童以及随访期间检测的所有未感染儿童的血清中均未发现针对HIV-1的IgA特异性抗体。由于母体IgA不会穿过胎盘，婴儿血清中IgA的检测表明存在HIV-1感染。（摘要截选至250字）
