Fusek M, Smith E A, Monod M, Foundling S I
Laboratory of Protein Crystallography, Oklahoma Medical Research Foundation, Oklahoma City 73104.
FEBS Lett. 1993 Jul 19;327(1):108-12. doi: 10.1016/0014-5793(93)81050-a.
We have isolated and characterized a second aspartic proteinase secreted by the CHUV E-18 strain of Candida parapsilosis. This proteinase is produced at a level corresponding to approximately 25% of the production of the main proteinase described earlier [1]. This minor proteinase has similar molecular weight and pH optimum but differs in the isoelectric point and in the specificity when compared with the major secreted form. The determination of the amino terminal amino acid sequence identified this minor form of Candida parapsilosis aspartic proteinase as a protein which corresponds to the sequence deduced from genomic DNA originally reported as a pseudogene [1]. We conclude that strain CHUV E-18 of Candida parapsilosis expresses and secretes two different aspartic proteinases.
我们已经分离并鉴定了近平滑念珠菌CHUV E - 18菌株分泌的第二种天冬氨酸蛋白酶。这种蛋白酶的产生水平约相当于之前描述的主要蛋白酶产量的25%[1]。与主要分泌形式相比，这种次要蛋白酶具有相似的分子量和最适pH，但等电点和特异性有所不同。氨基末端氨基酸序列的测定确定这种近平滑念珠菌天冬氨酸蛋白酶的次要形式是一种与最初报道为假基因的基因组DNA推导序列相对应的蛋白质[1]。我们得出结论，近平滑念珠菌CHUV E - 18菌株表达并分泌两种不同的天冬氨酸蛋白酶。
