Sono E, Masuda T, Takesako K, Kato I, Uchida K, Murayama S Y, Yamaguchi H
Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Shiga, Japan.
Microbiol Immunol. 1992;36(10):1099-104. doi: 10.1111/j.1348-0421.1992.tb02113.x.
Acid proteinases secreted by Candida tropicalis and C. parapsilosis were newly isolated. Their physico-chemical and enzymatic properties of molecular weight, pH stability, isoelectric points, specific activity, and N-terminal amino acid sequences were determined and compared with those of a C. albicans acid proteinase. The two acid proteinases secreted by C. parapsilosis were found to be new enzymes in their molecular weights. The acid proteinases from C. tropicalis and C. parapsilosis showed lower activity at neutral pH, less resistance to neutral and alkaline pH than that from C. albicans, and a half or a third of the specific activity of the C. albicans enzyme. These differences seemed to be associated with the difference of pathogenesis between Candida species. Of the 31 N-terminal amino acids, the enzymes of these three Candida species revealed 12 homologous amino acids.
热带假丝酵母和近平滑假丝酵母分泌的酸性蛋白酶被新分离出来。测定了它们的分子量、pH稳定性、等电点、比活性和N端氨基酸序列等理化及酶学性质，并与白色念珠菌酸性蛋白酶的这些性质进行了比较。发现近平滑假丝酵母分泌的两种酸性蛋白酶在分子量方面是新的酶。热带假丝酵母和近平滑假丝酵母的酸性蛋白酶在中性pH下活性较低，对中性和碱性pH的耐受性低于白色念珠菌的酸性蛋白酶，且比活性仅为白色念珠菌酶的一半或三分之一。这些差异似乎与念珠菌属之间致病机制的差异有关。在31个N端氨基酸中，这三种念珠菌的酶显示出12个同源氨基酸。
