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噬菌体7-7-1吸附到羽扇豆根瘤菌H13-3的复合鞭毛上。

Bacteriophage 7-7-1 adsorbs to the complex flagella of Rhizobium lupini H13-3.

作者信息

Lotz W, Acker G, Schmitt R

出版信息

J Gen Virol. 1977 Jan;34(1):9-17. doi: 10.1099/0022-1317-34-1-9.

DOI:10.1099/0022-1317-34-1-9
PMID:833579
Abstract

Bacteriophage 7-7-1 is shown to adsorb specifically to the complex flagella of its host Rhizobium lupini H13-3. Deflagellation of motile cells before the addition of phage leads to a complete inhibition of phage propagation for at least 60 min. Among phage-resistant mutants, many non-motile (mot) and non-flagellated (fla) derivatives of R. lupini H13-3 have been selected. Electron microscopic observations indicate that bacteriophage 7-7-1 attaches with its short tail fibres to the conspicuous helical filament of R. lupini flagells. This attachment is reversible; irreversible phage adsorption takes place at the flagellar base. It is postulated that phage 7-7-1 moves along the rotating flagellum towards a final receptor next to the insertion site of the flagellum, where tail contraction and injection of phage nucleic acid occurs.

摘要

噬菌体7-7-1被证明能特异性吸附于其宿主羽扇豆根瘤菌H13-3的复合鞭毛上。在添加噬菌体之前对运动性细胞进行鞭毛去除会导致噬菌体繁殖至少60分钟完全受到抑制。在抗噬菌体突变体中,已筛选出许多羽扇豆根瘤菌H13-3的非运动型(mot)和无鞭毛型(fla)衍生物。电子显微镜观察表明,噬菌体7-7-1通过其短尾丝附着于羽扇豆根瘤菌鞭毛明显的螺旋丝上。这种附着是可逆的;噬菌体在鞭毛基部发生不可逆吸附。据推测,噬菌体7-7-1沿着旋转的鞭毛向鞭毛插入位点旁边的最终受体移动,在那里发生尾部收缩和噬菌体核酸注入。

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