Tetz V V, Rybalchenko O V, Savkova G A
St. Petersburg Pavlov Medical Institute, Russia.
J Basic Microbiol. 1993;33(2):131-9. doi: 10.1002/jobm.3620330210.
Electron microscopic examination of the morphology of normal Escherichia coli MC4100 and Shigella flexneri cells and their htpR-defective recombinants has shown the presence in the latter of noncrystalline granules after growth at 37 degrees C. The defect of the htpR gene leads to some changes in ultrastructure of bacterial colonies and the intensification of the processes of development and aging of colonies as integral structures. These phenomena are manifested by early formation of intercellular contacts and thick surface films as well as the enhanced destruction of microorganisms. The recombinant Shigella strain grown at 30 degrees C retained its ability to provoke keratoconjunctivitis.
对正常大肠杆菌MC4100和福氏志贺氏菌细胞及其htpR缺陷型重组体的形态进行电子显微镜检查发现，后者在37℃生长后存在非晶质颗粒。htpR基因的缺陷导致细菌菌落超微结构的一些变化以及作为整体结构的菌落发育和老化过程的加剧。这些现象表现为细胞间接触的早期形成和厚表面膜的形成以及微生物破坏的增强。在30℃下生长的重组志贺氏菌菌株保留了引发角膜结膜炎的能力。
