Ellerbe P, Long T, Welch M J
Organic Analytical Research Division, National Institute of Standards and Technology, Technology Administration, Department of Commerce, Gaithersburg, MD 20899.
J Anal Toxicol. 1993 May-Jun;17(3):165-70. doi: 10.1093/jat/17.3.165.
The concentrations of amphetamine and methamphetamine in a new lyophilized human urine reference material (RM) were determined at the National Institute of Standards and Technology, in cooperation with the College of American Pathologists. In order to minimize the possibility of undetected bias, two different methods were developed. Both methods were based on isotope-dilution gas chromatography/mass spectrometry (GC/MS) with a deuterium-labeled standard. The first method used a solid phase extractor (SPE) to isolate the analytes, followed by forming the heptafluorobutyryl (HFB) derivatives for measurement by GC/MS. The second method used another kind of SPE to isolate the analytes, followed by forming the N-trifluoroacetyl-1-prolyl chloride (TPC) derivatives for measurement by GC/MS. The RM consists of three levels of amphetamine, three levels of methamphetamine, and a blank. For amphetamine, the concentrations are 291, 558, and 1081 ng/mL of human urine. For methamphetamine, the concentrations are 311, 573, and 1137 ng/mL of human urine.
在美国病理学家学会的合作下，美国国家标准与技术研究院测定了一种新型冻干人尿参考物质（RM）中苯丙胺和甲基苯丙胺的浓度。为了尽量减少未检测到的偏差的可能性，开发了两种不同的方法。两种方法均基于带有氘标记标准品的同位素稀释气相色谱/质谱联用（GC/MS）。第一种方法使用固相萃取器（SPE）分离分析物，然后形成七氟丁酰（HFB）衍生物，通过GC/MS进行测量。第二种方法使用另一种SPE分离分析物，然后形成N-三氟乙酰-1-脯氨酰氯（TPC）衍生物，通过GC/MS进行测量。该参考物质包含三个浓度水平的苯丙胺、三个浓度水平的甲基苯丙胺以及一个空白样本。对于苯丙胺，其浓度分别为每毫升人尿291、558和1081纳克。对于甲基苯丙胺，其浓度分别为每毫升人尿311、573和1137纳克。
