Kim D, Weaver R F
Department of Biochemistry, University of Kansas, Lawrence 66045-2106.
Virology. 1993 Aug;195(2):587-95. doi: 10.1006/viro.1993.1410.
The protein tyrosine/serine phosphatase (PTPase) gene of the Autographa californica nuclear polyhedrosis virus yields two major transcripts of approximate sizes 3.1 and 3.9 kb. Both of these are very late transcripts, which accumulate to maximal levels more than 30 hr postinfection. The smaller transcript initiates at the T of an ATAAG sequence that lies 22 bp upstream of the putative start codon of the gene. The larger transcript initiates at the first A of a TTAAG sequence that lies approximately 798 bp further upstream. Thus, the larger transcript is made by transcribing through the entire hr1 region, which lies just 60 bp upstream of the putative translation start site. We have expressed the product of this gene as a fusion protein with glutathione-S-transferase and have shown that it has PTPase activity similar to that of the vaccinia virus H1 gene product: It dephosphorylates both protein phosphotyrosines and phosphoserines/phosphothreonines, and it is inhibited by vanadate, but not by okadaic acid.
苜蓿银纹夜蛾核型多角体病毒的蛋白酪氨酸/丝氨酸磷酸酶（PTPase）基因产生两种主要转录本，大小约为3.1 kb和3.9 kb。这两种都是极晚期转录本，在感染后30多小时积累到最高水平。较小的转录本从位于该基因推定起始密码子上游22 bp处的ATAAG序列的T处起始。较大的转录本从位于约798 bp更上游的TTAAG序列的第一个A处起始。因此，较大的转录本是通过转录整个hr1区域产生的，该区域仅位于推定翻译起始位点上游60 bp处。我们已将该基因的产物表达为与谷胱甘肽-S-转移酶的融合蛋白，并表明它具有与痘苗病毒H1基因产物相似的PTPase活性：它能使蛋白磷酸酪氨酸和磷酸丝氨酸/磷酸苏氨酸去磷酸化，并且它受钒酸盐抑制，但不受冈田酸抑制。
