McGregor J M, Yu C C, Dublin E A, Barnes D M, Levison D A, MacDonald D M
Department of Dermatology, Guy's Hospital, London, U.K.
Br J Dermatol. 1993 Jun;128(6):606-11. doi: 10.1111/j.1365-2133.1993.tb00253.x.
Expression of the tumour suppressor protein, p53, was determined in 77 cutaneous melanocytic lesions, and in five lymph node metastases from malignant melanoma, in an immunohistochemical study employing CM-1, an antiserum raised against recombinant human p53 protein. Because wild-type p53 protein is rapidly degraded in normal cells, p53 immunoreactivity suggests the presence of an abnormally stable p53 protein. This may occur through either post-translational mechanisms or gene mutation. A highly significant correlation was found between p53 immunoreactivity and malignancy in melanocytic lesions (P < 0.0001). Overall, p53 immunoreactivity was observed in 63% of tumour specimens examined, but not in benign melanocytic naevi, although occasional foci of weak nuclear p53 immunoreactivity were observed in a minority of dysplastic naevi and a solitary Spitz naevus. A significant correlation was also found between strong p53 immunoreactivity and malignant melanomas associated with a poor prognosis (P = 0.008). These data suggest an important role for p53 tumour suppressor protein in the biology of human cutaneous malignant melanoma.
在一项免疫组织化学研究中，使用针对重组人p53蛋白产生的抗血清CM-1，对77例皮肤黑素细胞病变以及5例恶性黑素瘤的淋巴结转移灶进行了肿瘤抑制蛋白p53的表达检测。由于野生型p53蛋白在正常细胞中会迅速降解，p53免疫反应性提示存在异常稳定的p53蛋白。这可能通过翻译后机制或基因突变发生。在黑素细胞病变中，发现p53免疫反应性与恶性程度之间存在高度显著的相关性（P < 0.0001）。总体而言，在所检查的肿瘤标本中，63%观察到p53免疫反应性，但在良性黑素细胞痣中未观察到，尽管在少数发育异常痣和一个孤立的Spitz痣中偶尔观察到弱核p53免疫反应性灶。在与预后不良相关的恶性黑素瘤中，强p53免疫反应性之间也发现了显著相关性（P = 0.008）。这些数据表明p53肿瘤抑制蛋白在人类皮肤恶性黑素瘤生物学中起重要作用。
