Lee C S, Pirdas A, Lee M W
Department of Pathology, University of Melbourne, Parkville, Victoria, Australia.
Australas J Dermatol. 1995 Nov;36(4):192-5. doi: 10.1111/j.1440-0960.1995.tb00971.x.
p53 protein immunoreactivity was investigated in cutaneous Spitz naevi (n = 7), superficial spreading (n = 21) and nodular (n = 6) melanomas using the monoclonal antibody DO-7. Nuclear immunostaining for p53 protein was observed in 41% of all the melanomas but not in any of the Spitz naevi studied. This difference in p53 immunoreactivity appeared statistically significant (P = 0.0001; chi 2 test). There were also differences in p53 immunoreactivity between superficial spreading and nodular melanomas (P = 0.001, chi 2 test), where up to 38% of superficial spreading melanomas and 50% of nodular melanomas had demonstrated p53 nuclear immunostaining. An apparently significant correlation was observed between melanomas with poor prognostic histological indices and p53 immunoreactivity (P = 0.0016; chi 2 test). In conclusion, increased p53 protein immunoreactivity is found in melanomas and is associated with poorer prognosis. The differences in p53 immunohistological expression between cutaneous melanomas and Spitz naevi suggest that alterations of the protein may be important in the pathogenesis of the tumour.
使用单克隆抗体DO-7对皮肤Spitz痣（n = 7）、浅表扩散型（n = 21）和结节型（n = 6）黑色素瘤中的p53蛋白免疫反应性进行了研究。在所有黑色素瘤中，41%观察到p53蛋白的核免疫染色，但在所研究的任何Spitz痣中均未观察到。p53免疫反应性的这种差异具有统计学意义（P = 0.0001；卡方检验）。浅表扩散型和结节型黑色素瘤之间的p53免疫反应性也存在差异（P = 0.001，卡方检验），其中高达38%的浅表扩散型黑色素瘤和50%的结节型黑色素瘤表现出p53核免疫染色。在具有不良预后组织学指标的黑色素瘤和p53免疫反应性之间观察到明显的相关性（P = 0.0016；卡方检验）。总之，在黑色素瘤中发现p53蛋白免疫反应性增加，且与较差的预后相关。皮肤黑色素瘤和Spitz痣之间p53免疫组织化学表达的差异表明，该蛋白的改变可能在肿瘤发病机制中起重要作用。
