Shih Y L, Lo S J
Graduate Institute of Microbiology and Immunology, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
Cell Biol Int. 1993 May;17(5):533-42. doi: 10.1006/cbir.1993.1095.
Treatment of GMM-1 (a goldfish melanocytoma cell line) cells with epinephrine induced a rapid cell expansion (flattening of cells, extension and broadening of cellular processes) similar to the effect of dexamethasone reported previously (Shih et al., 1990). Studies on the possible involvement of secondary messengers in cell expansion indicated that (i) both 8-bromo-CAMP and forskolin caused cell shrinking (the opposite of cell expansion); (ii) TPA also caused cell shrinking; (iii) phospholipid derivatives, such as 1,2-dioctanoyl-sn-glycerol, lysophosphatidic acid, and arachidonic acid caused cell expansion; and (iv) EGTA (calcium chelator) and nifedipine (calcium channel blocker) inhibited the effect of epinephrine. Together with the previous findings, these observations indicate that epinephrine and dexamethasone may share a common pathway in triggering an external calcium influx to cause cell expansion. The results of the effects of epinephrine agonists and antagonists, together with those of other workers, also show that there are multiple isoforms of adrenoceptor in the goldfish.
用肾上腺素处理金鱼黑素细胞瘤细胞系GMM - 1会诱导细胞迅速扩张（细胞变扁平、细胞突起伸展并变宽），这类似于先前报道的地塞米松的作用（Shih等人，1990年）。对细胞扩张中可能涉及的第二信使的研究表明：（i）8 - 溴 - CAMP和福斯可林均导致细胞收缩（与细胞扩张相反）；（ii）佛波酯也导致细胞收缩；（iii）磷脂衍生物，如1,2 - 二辛酰 - sn - 甘油、溶血磷脂酸和花生四烯酸导致细胞扩张；以及（iv）乙二醇双（2 - 氨基乙醚）四乙酸（钙螯合剂）和硝苯地平（钙通道阻滞剂）抑制肾上腺素的作用。与先前的发现一起，这些观察结果表明肾上腺素和地塞米松在触发细胞外钙内流以引起细胞扩张方面可能共享一条共同途径。肾上腺素激动剂和拮抗剂的作用结果，连同其他研究人员的结果，也表明金鱼中存在多种肾上腺素能受体亚型。
