Oka K, Hirano T, Homma M, Ishii H, Murakami K, Mogami S, Motizuki A, Morita H, Takeya K, Itokawa H
Tokyo College of Pharmacy, Japan.
Chem Pharm Bull (Tokyo). 1993 May;41(5):1000-2. doi: 10.1248/cpb.41.1000.
We optimized HPLC conditions for the first time to achieve satisfactory separation of six surfactins, two of which were novel, isolated from Bacillus subtilis natto. Peptide sequences were analyzed by sophisticated MS-MS analysis, performed with a pair of two surfactins with different fatty acid substitutions. Fragment peaks were divided into two series; one was a series of the ions which carried fatty acid, and thus were characterized by the fatty acid mass difference, and the other was a series of the ions which consisted of pure peptide and thus showed a superimposable pattern between a pair of surfactins.
我们首次优化了高效液相色谱条件，以实现对六种表面活性素的满意分离，其中两种是从纳豆芽孢杆菌中分离出的新型表面活性素。通过精密的串联质谱分析对肽序列进行了分析，该分析是针对一对具有不同脂肪酸取代的表面活性素进行的。碎片峰分为两个系列；一个是携带脂肪酸的离子系列，因此以脂肪酸质量差异为特征，另一个是由纯肽组成的离子系列，因此在一对表面活性素之间呈现出可叠加的模式。
