枯草芽孢杆菌中源自pSM19035的复制子的功能分析。

Functional analysis of pSM19035-derived replicons in Bacillus subtilis.

作者信息

Ceglowski P, Lurz R, Alonso J C

机构信息

Max-Planck-Institut für Molekulare Genetik, Berlin, FRG.

出版信息

FEMS Microbiol Lett. 1993 May 15;109(2-3):145-50. doi: 10.1016/0378-1097(93)90011-p.

DOI:10.1016/0378-1097(93)90011-p

PMID:8339905

Abstract

We determined the effect of various Bacillus subtilis dna(Ts) mutations on pSM19035 replication. At non-permissive temperature, plasmid replication depends on the dnaB, dnaC, dnaG and dnaI initiation replication products, but does not require the dnaA function. DNA elongation is performed by DNA polymerase III, since replication is blocked at non-permissive temperature in dna(Ts) mutants impaired in different subunits of the enzyme. Single-stranded plasmid replication intermediates, corresponding to the leading strand, accumulate in the dnaD strain. On the basis of electron microscopic analysis of replication intermediates, we confirmed that the plasmid replicates unidirectionally by a theta mechanism.

摘要

我们确定了各种枯草芽孢杆菌dna(Ts)突变对pSM19035复制的影响。在非允许温度下，质粒复制依赖于dnaB、dnaC、dnaG和dnaI起始复制产物，但不需要dnaA功能。DNA延伸由DNA聚合酶III进行，因为在非允许温度下，该酶不同亚基受损的dna(Ts)突变体中复制被阻断。对应于前导链的单链质粒复制中间体在dnaD菌株中积累。基于对复制中间体的电子显微镜分析，我们证实该质粒通过θ机制单向复制。