Takahashi H
Department of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1993 Jul;68(4):485-95.
Human T-cell leukemia (or lymphotrophic) virus type II (HTLV-II) was isolated from eight HTLV seropositive patients. Restriction endonuclease mapping of the proviruses demonstrated consistent differences among isolates, and two distinct physical map patterns were observed. These studies have provided evidence for existence of two related, but distinct molecular subtype of HTLV-II, which are designated HTLV-II a and HTLV-II b. Between the two subtypes, the gag region encoding the p19 protein of HTLV-II b isolates contained a 66bp deletion in addition to single nucleotide differences. Immunoblotting methods employing sera from infected patients with each subtype failed to demonstrate any antigenic differences when recombinant gag p19 protein of each subtype was used as antigen. However, similar assays using recombinant HTLV-I and HTLV-II p19 proteins were able to differentiate the two viruses. ELISA using the synthetic peptides corresponding the deleted region gave the negative results for HTLV-II b (0/6) in contrast to the high positivity for HTLV-II a (6/10), which would provide an initial screening method for differentiation of the HTLV-II subtypes.
人类T细胞白血病（或嗜淋巴细胞）病毒II型（HTLV-II）是从8名HTLV血清反应阳性患者中分离出来的。对前病毒进行限制性内切酶图谱分析显示，各分离株之间存在一致的差异，并且观察到两种不同的物理图谱模式。这些研究为HTLV-II存在两种相关但不同的分子亚型提供了证据，这两种亚型被命名为HTLV-II a和HTLV-II b。在这两种亚型之间，编码HTLV-II b分离株p19蛋白的gag区域除了单核苷酸差异外，还存在一个66bp的缺失。当使用各亚型的重组gag p19蛋白作为抗原时，采用来自每种亚型感染患者血清的免疫印迹方法未能显示出任何抗原差异。然而，使用重组HTLV-I和HTLV-II p19蛋白进行的类似检测能够区分这两种病毒。使用与缺失区域对应的合成肽进行的ELISA检测，HTLV-II b的结果为阴性（0/6），而HTLV-II a的阳性率很高（6/10），这将为区分HTLV-II亚型提供一种初步筛选方法。
