[增强化学发光免疫分析法测定庆大霉素的研究]

[Study on determination of gentamycin by enhanced chemiluminescent immunoassay].

作者信息

Deng A, Yang X, Yang Y, Yang H

出版信息

Hua Xi Yi Ke Da Xue Xue Bao. 1993 Mar;24(1):101-3.

Abstract

Gentamycin was determined by solid antigen competition inhibition method of chemiluminescet immunoassay, in which luminol-(p-hydroxydiphenyl)-H2O2 was used a the chemiluminescence system and p-hydroxydiphenyl as the chemiluminescent enhancer. Solid antigen was synthesized by a method in which gentamycin was immobilized covalently on microcrystalline cellulose previously activated by 1,1'-carbonyldiimidazole. The rabbit anti-gentamycin IgG and the HRP-donkey anti-rabbit IgG were used as the first antibody and labelled second antibody, respectively. The linear range of the gentamycin (with blank serum) was 0.03-1 micrograms/tube and the correlation coefficient of the log-log regression equation [r[ was 0.981 +/- 0.016 with a limit of detection 3.3-11.4 ng/tube and the recovery of gentamycin 88.2 +/- 4.5%.

摘要

采用化学发光免疫分析的固相抗原竞争抑制法测定庆大霉素，以鲁米诺 -（对羟基二苯基）-H₂O₂作为化学发光体系，对羟基二苯基作为化学发光增强剂。固相抗原通过将庆大霉素共价固定在预先用1,1'-羰基二咪唑活化的微晶纤维素上的方法合成。兔抗庆大霉素IgG和辣根过氧化物酶标记的驴抗兔IgG分别用作第一抗体和标记二抗。庆大霉素（含空白血清）的线性范围为0.03 - 1微克/管，对数 - 对数回归方程的相关系数[r]为0.981±0.016，检测限为3.3 - 11.4纳克/管，庆大霉素的回收率为88.2±4.5%。