Bakes D M, Turner N D, Gordon B H, Hiley M P, Walther B, Lucas C
Servier Research and Development Ltd., Fulmer, Slough, UK.
J Chromatogr. 1993 May 19;615(1):117-26. doi: 10.1016/0378-4347(93)80297-h.
An extraction method has been developed using benzene sulphonyl cation-exchange sample preparation cartridges and reversed-phase high-performance liquid chromatography with ultraviolet detection for the measurement of S9788, a drug to reverse resistance to anticancer agents, in plasma and serum. This includes a toxicokinetic assay which has a mean precision and accuracy of 11.7% and 7.9%, respectively, over the range 10-1000 ng ml-1 and a quantification limit of 10 ng ml-1 and a more sensitive pharmacokinetic procedure with a mean precision and accuracy of 5.0% and 7.9%, respectively, over the range 1-500 ng ml-1 and a quantification limit of 1 ng ml-1. The specificity of the procedure has been demonstrated by mass and ultraviolet spectrometry, and linearity, precision, accuracy, recovery and sensitivity have been established. The assays have been successfully applied to toxicokinetic and pharmacokinetic studies.
已开发出一种萃取方法，该方法使用苯磺酰阳离子交换样品制备柱以及带有紫外检测的反相高效液相色谱法，用于测定血浆和血清中S9788（一种逆转抗癌药耐药性的药物）。这包括一项毒代动力学分析，在10 - 1000 ng/ml范围内，其平均精密度和准确度分别为11.7%和7.9%，定量限为10 ng/ml；还有一种更灵敏的药代动力学方法，在1 - 500 ng/ml范围内，其平均精密度和准确度分别为5.0%和7.9%，定量限为1 ng/ml。该方法的特异性已通过质谱和紫外光谱法得到证明，并且已确定了线性、精密度、准确度、回收率和灵敏度。这些分析方法已成功应用于毒代动力学和药代动力学研究。
