Hisatomi K, Maruyama H, Isomura T, Aoyagi S, Kosuga K, Ohishi K
Second Department of Surgery, Kurume University School of Medicine, Japan.
Jpn Circ J. 1993 Jun;57(6):558-62. doi: 10.1253/jcj.57.558.
We discuss here the effect of the addition of trinitroglycerin to the cardioplegic solution on the isolated rat heart after eight hours of storage. This effect was evaluated by measuring cardiac function as well as myocardial calcium and enzymes. Male Wistar rats were divided into three groups based on the concentration of trinitroglycerine in the cardioplegic solution. In the control group, the fluid used was a crystalloid cardioplegic solution (K+ 25 mEq/L) without trinitroglycerin and for groups I and II, trinitroglycerin was added at concentrations of 2 micrograms/ml and 5 micrograms/ml, respectively. All hearts were arrested with the cardioplegic solution at 4 degrees C and then immersed for eight hours in Euro-Collins' solution at 4 degrees C. The postperfusion coronary flow showed higher rates for groups I and II than for the control group (p < 0.05), while the difference between groups I and II was not significant. In group I, the total concentrations of creatine kinase-MB, lactate and malondialdehyde after reperfusion showed the lowest levels; this group also had the lowest content of myocardial calcium. These results indicate that the addition of nitroglycerin, especially at a concentration of 2 micrograms/ml, to the cardioplegic solution elicits better cardiac function for immersed rat heart.
我们在此讨论在心脏停搏液中添加三硝酸甘油酯对储存8小时后的离体大鼠心脏的影响。通过测量心脏功能以及心肌钙和酶来评估这种影响。根据心脏停搏液中三硝酸甘油酯的浓度，将雄性Wistar大鼠分为三组。对照组使用的液体是不含三硝酸甘油酯的晶体心脏停搏液（K + 25 mEq/L），I组和II组分别添加浓度为2微克/毫升和5微克/毫升的三硝酸甘油酯。所有心脏均用心脏停搏液在4℃下停搏，然后在4℃下浸入Euro-Collins溶液中8小时。灌注后冠状动脉流量显示I组和II组高于对照组（p < 0.05），而I组和II组之间的差异不显著。在I组中，再灌注后肌酸激酶-MB、乳酸和丙二醛的总浓度显示为最低水平；该组心肌钙含量也最低。这些结果表明，在心脏停搏液中添加硝酸甘油，尤其是浓度为2微克/毫升时，对浸入的大鼠心脏能引发更好的心脏功能。
