Kokaia Z, Bengzon J, Metsis M, Kokaia M, Persson H, Lindvall O
Department of Neurology, University Hospital, Lund, Sweden.
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6711-5. doi: 10.1073/pnas.90.14.6711.
Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are neuronal survival molecules which utilize the Trk family of tyrosine kinase receptors. Using double-label in situ hybridization, we demonstrate that mRNAs for BDNF and its high-affinity receptor TrkB are coexpressed in hippocampal and cortical neurons. Also, a large number of neurons in these areas coexpress NGF and BDNF mRNAs. Epileptic seizures lead to increased levels of both BDNF/TrkB and NGF/BDNF mRNAs in double-labeled cells. Our results show that individual neurons of the central nervous system can coexpress neurotrophins and their receptors and produce two neurotrophic factors. These factors could support neuronal survival after brain insults, not only via retrograde transport but also through autocrine mechanisms.
神经生长因子（NGF）和脑源性神经营养因子（BDNF）是利用酪氨酸激酶受体Trk家族的神经元存活分子。通过双标记原位杂交，我们证明BDNF及其高亲和力受体TrkB的mRNA在海马和皮质神经元中共同表达。此外，这些区域的大量神经元共同表达NGF和BDNF的mRNA。癫痫发作会导致双标记细胞中BDNF/TrkB和NGF/BDNF的mRNA水平升高。我们的结果表明，中枢神经系统的单个神经元可以共同表达神经营养因子及其受体，并产生两种神经营养因子。这些因子不仅可以通过逆行运输，还可以通过自分泌机制来支持脑损伤后神经元的存活。
