Miranda R C, Sohrabji F, Toran-Allerand C D
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6439-43. doi: 10.1073/pnas.90.14.6439.
Development and survival of neurons in the central nervous system are dependent on the activity of a variety of endogenous neurotrophic agents. Using combined isotopic and nonisotopic in situ hybridization histochemistry, we have found that subsets of neurons within the developing forebrain coexpress the mRNAs for both neurotrophins (nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3) and their receptors (p75NGFR, TrkA, and TrkB). The colocalization of mRNA for neurotrophin receptors and their ligands in presumptive neurotrophin target neurons suggests the potential for autocrine and paracrine mechanisms of action during development. Such mechanisms may ensure the onset of differentiation and survival of specific subsets of neurons prior to and following target innervation.
中枢神经系统中神经元的发育和存活依赖于多种内源性神经营养因子的活性。利用同位素和非同位素原位杂交组织化学相结合的方法，我们发现发育中的前脑内的神经元亚群同时表达神经营养因子（神经生长因子、脑源性神经营养因子和神经营养因子3）及其受体（p75NGFR、TrkA和TrkB）的mRNA。神经营养因子受体及其配体的mRNA在假定的神经营养因子靶神经元中的共定位表明在发育过程中存在自分泌和旁分泌作用机制的可能性。这些机制可能确保在靶神经支配之前和之后特定神经元亚群的分化和存活的开始。
