羽扇豆根瘤菌中通过接合形成部分二倍体克隆。

Formation of merodiploid clones by cojugation in Rhizobium lupini.

作者信息

Heumann W, Springer R

出版信息

Mol Gen Genet. 1977 Jan 7;150(1):73-9. doi: 10.1007/BF02425327.

Abstract

Off the transconjugants formed in the R. lupini conjugation 0.5 to 5% are merodiploids. When two differently pigmented parents are used in the crossing experiment the diploid transconjugants by their additive pigmentation type. The segregation patterns of these diploid clones were analyzed. The results are in agreement with the theory that the exogenotic donor DNA can be integrated at different sites of the homologous recipient chromosomal region forming a tandem sequence. Consequently the segregants of these merodiploid clones are formed by endochromosomal recombination.

摘要

在羽扇豆属接合过程中形成的转接合子中，0.5%至5%是部分二倍体。当在杂交实验中使用两个色素沉着不同的亲本时，二倍体转接合子具有加性色素沉着类型。分析了这些二倍体克隆的分离模式。结果与外源供体DNA可整合到同源受体染色体区域的不同位点形成串联序列的理论一致。因此，这些部分二倍体克隆的分离子是通过染色体内重组形成的。