Block T, Treiber U, Geffken B, Vogel M, Hanauske A R, Schmid F, Hartung R, Busch R
Department of Urology, Technische Universität, Munich, Germany.
Urol Res. 1993 May;21(3):217-21. doi: 10.1007/BF00590039.
In five of eight human transitional carcinoma cell (TCC) lines a proliferative response has been reported during exposure to interleukin-3 (IL-3), granulocyte-macrophage colony stimulating factor (GM-CSF) and granulocyte colony stimulating factor (G-CSF). To elucidate possible growth-modulating effects of these factors combined with clinically relevant antineoplastic agents, cells of the human TCC lines EJ28 and T24 were exposed to methotrexate (MTX), vinblastine (VBL), doxorubicin (DXR) and cisplating (CDDP) with and without single or continuous exposure to IL-3, GM-CSF and G-CSF at concentrations of 1-100 ng/ml. Compared with cells exposed only to chemotherapy, significant inhibitory effects occurred as a result of continuous exposure to IL-3 or GM-CSF at the highest activities with CDDP and MTX in the T24 and EJ28 lines; continuous G-CSF administration (100 ng/ml) in combination with MTX led to significant growth inhibition in the EJ28 line. In contrast, no significant growth modulation was found on combined administration of DXR or VBL with any one of the three colony stimulating factors tested.
据报道，在8种人移行细胞癌（TCC）细胞系中，有5种在暴露于白细胞介素-3（IL-3）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）期间出现增殖反应。为了阐明这些因子与临床相关抗肿瘤药物联合使用时可能的生长调节作用，将人TCC细胞系EJ28和T24的细胞暴露于甲氨蝶呤（MTX）、长春碱（VBL）、阿霉素（DXR）和顺铂（CDDP），同时分别以1-100 ng/ml的浓度单独或连续暴露于IL-3、GM-CSF和G-CSF。与仅接受化疗的细胞相比，在T24和EJ28细胞系中，在最高活性下连续暴露于IL-3或GM-CSF并联合CDDP和MTX，产生了显著的抑制作用；连续给予G-CSF（100 ng/ml）并联合MTX导致EJ28细胞系显著生长抑制。相比之下，未发现DXR或VBL与所测试的三种集落刺激因子中的任何一种联合给药时有显著的生长调节作用。
