大麦（Hordeum vulgare）线粒体ATP合酶亚基9的RNA编辑

RNA editing of the barley (Hordeum vulgare) mitochondrial ATP synthase subunit 9.

作者信息

Rish S Y, Breiman A

机构信息

Department of Botany, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.

出版信息

Plant Mol Biol. 1993 Jul;22(4):711-4. doi: 10.1007/BF00047412.

DOI:10.1007/BF00047412

PMID:8343607

Abstract

The barley (Hordeum vulgare) FO-ATP synthase subunit 9 (atp9) was isolated from the mitochondrial DNA. Two copies of atp9 are present in barley mitochondria and 2 major transcripts of 2.7 kb and 1.2 kb were detected on RNA blots. RNA editing as C-to-U conversion occurs in barley atp9 in seven positions. Five of these seven positions lead to an amino acid change whereas two conversions are silent. The editing positions of barley were found to be identical to those of wheat. Whereas in wheat, Oenothera and Petunia all the atp9 transcripts were fully edited, 24% of the barley cDNA clones were partially edited as compared to 10% partially edited clones in wheat.

摘要

从线粒体DNA中分离出大麦（Hordeum vulgare）的FO-ATP合酶亚基9（atp9）。大麦线粒体中存在两个atp9拷贝，在RNA印迹上检测到2.7 kb和1.2 kb的两个主要转录本。大麦atp9中发生了七个位置的RNA编辑，即C到U的转换。这七个位置中的五个导致氨基酸变化，而两个转换是沉默的。发现大麦的编辑位置与小麦的相同。在小麦、月见草和矮牵牛中，所有的atp9转录本都被完全编辑，而与小麦中10%的部分编辑克隆相比，24%的大麦cDNA克隆是部分编辑的。