Wintz H, Hanson M R
Section of Genetics and Development, Cornell University, Ithaca, NY.
Curr Genet. 1991 Jan;19(1):61-4. doi: 10.1007/BF00362089.
Analysis of the cDNA of the atp9-1 gene transcript from petunia mitochondria has revealed that ten C residues of the gene sequence are edited into U in the mRNA. Seven of these edits result in amino acid changes and one introduces a stop codon before the end of the open reading frame predicted from the gene sequence. The resulting protein is better conserved when compared to the same protein in other organisms. Comparison of the edited petunia sequence with other plant mitochondrial atp9 gene sequences idicates variation in the number and positions of edits required to obtain the same amino acids in ATP9 polypeptides of higher plants.
对矮牵牛属线粒体中atp9 - 1基因转录本的cDNA分析表明,该基因序列中的10个C残基在mRNA中被编辑为U。其中7个编辑导致氨基酸变化,1个在基因序列预测的开放阅读框末端之前引入了一个终止密码子。与其他生物体中的相同蛋白质相比,所得蛋白质的保守性更好。将编辑后的矮牵牛序列与其他植物线粒体atp9基因序列进行比较,结果表明,在高等植物ATP9多肽中获得相同氨基酸所需的编辑数量和位置存在差异。