Vassilevskaia T D, Ricardo C P, Rodrigues-Pousada C
Instituto Gulbenkian de Ciência, Laboratório de Genética Molecular, Oeiras, Portugal.
Plant Mol Biol. 1993 Jul;22(4):715-8. doi: 10.1007/BF00047413.
Genomic lambda-Dash library constructed from Lupinus albus nuclear DNA was screened using a fragment of the beta-tubulin cDNA (beta 8-31) clone of Chlamydomonas reinhardtii as probe. One of the positive recombinant phages was isolated, subcloned and analysed by sequencing. We present here nucleotide and derived amino acid sequences of the beta-tubulin gene, designated as L beta 1 and identified by similarity with other beta-tubulins. The L beta 1-encoded protein reveals a very high degree of similarity with other plant tubulins and contains consensus sequences for binding guanine base, phosphate and Mg2+. Northern analysis of total RNA isolated from roots, leaves, flowers and pools revealed that Lupinus albus beta-tubulin genes are constitutively expressed in all studied plant tissues.
用莱茵衣藻β-微管蛋白cDNA（β8 - 31）克隆片段作为探针，筛选由白羽扇豆核DNA构建的基因组λ-Dash文库。分离出一个阳性重组噬菌体，进行亚克隆并测序分析。我们在此展示了β-微管蛋白基因的核苷酸和推导的氨基酸序列，命名为Lβ1，并通过与其他β-微管蛋白的相似性进行鉴定。Lβ1编码的蛋白质与其他植物微管蛋白具有高度相似性，并含有结合鸟嘌呤碱基、磷酸和Mg2 +的共有序列。对从根、叶、花和库中分离的总RNA进行Northern分析表明，白羽扇豆β-微管蛋白基因在所有研究的植物组织中组成性表达。
