弗林蛋白酶对肝细胞生长因子/分散因子受体的蛋白水解加工

Proteolytic processing of the hepatocyte growth factor/scatter factor receptor by furin.

作者信息

Komada M, Hatsuzawa K, Shibamoto S, Ito F, Nakayama K, Kitamura N

机构信息

Institute for Liver Research, Kansai Medical University, Osaka, Japan.

出版信息

FEBS Lett. 1993 Aug 9;328(1-2):25-9. doi: 10.1016/0014-5793(93)80958-w.

DOI:10.1016/0014-5793(93)80958-w

PMID:8344430

Abstract

The hepatocyte growth factor/scatter factor (HGF/SF) receptor consists of an alpha- and a beta-subunit, which are derived from a single-chain precursor by endoproteolytic processing. The precursor is not proteolytically processed in LoVo colon carcinoma cells. The uncleaved receptor immunopurified from the cells was cleaved in vitro by furin. Furthermore, the HGF/SF receptor was proteolytically processed in LoVo cells transfected with furin cDNA. These results indicate that furin is a processing endoprotease for the HGF/SF receptor. Tyrosine autophosphorylation of the uncleaved receptor was induced by HGF/SF, and the growth of the cells expressing the uncleaved receptor was stimulated by HGF/SF, indicating that the proteolytic processing of the receptor is not essential for the signal transduction of HGF/SF.

摘要

肝细胞生长因子/分散因子（HGF/SF）受体由α亚基和β亚基组成，它们通过内切蛋白水解加工从单链前体衍生而来。该前体在LoVo结肠癌细胞中未进行蛋白水解加工。从细胞中免疫纯化的未切割受体在体外被弗林蛋白酶切割。此外，在转染了弗林蛋白酶cDNA的LoVo细胞中，HGF/SF受体进行了蛋白水解加工。这些结果表明，弗林蛋白酶是HGF/SF受体的加工内切蛋白酶。未切割受体的酪氨酸自磷酸化由HGF/SF诱导，表达未切割受体的细胞生长受到HGF/SF的刺激，这表明受体的蛋白水解加工对于HGF/SF的信号转导并非必不可少。