Dimier I H, Bout D T
Unité de Recherche Université-INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques de Tours, Nouzilly, France.
Immunology. 1993 Jun;79(2):336-8.
The tachyzoites of the RH strain of Toxoplasma gondii invaded and replicated in cultured human umbilical vein endothelial cells (HUVEC). Co-treatment of the HUVEC with human recombinant interleukin-1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha) inhibited the replication of T. gondii. Growth of the parasite was measured in vitro by [3H]uracil incorporation assay 18 hr after infection. This assay showed that when cells were pretreated with IL-1 beta and TNF-alpha concentrations ranging from 1 to 100 U/ml, a high degree of inhibition was observed with a phenomenon of co-operation causing a dose-dependent inhibition of the replication of intracellular T. gondii. The mechanism(s) by which the inhibition of multiplication was effected remains to be elucidated.
刚地弓形虫RH株速殖子侵入培养的人脐静脉内皮细胞（HUVEC）并在其中复制。用人重组白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）共同处理HUVEC可抑制刚地弓形虫的复制。感染18小时后，通过[3H]尿嘧啶掺入试验在体外测定寄生虫的生长。该试验表明，当用浓度范围为1至100 U/ml的IL-1β和TNF-α预处理细胞时，观察到高度抑制，且存在协同作用现象，导致细胞内刚地弓形虫复制受到剂量依赖性抑制。抑制增殖的作用机制尚待阐明。
