Murdough P A, Pankey J W
Department of Animal and Food Science, University of Vermont, Burlington 05405.
J Dairy Sci. 1993 Jul;76(7):2033-8. doi: 10.3168/jds.s0022-0302(93)77537-2.
Fifty-seven teat dip formulations were tested for germicidal activity with an excised teat assay. Streptococcus agalactiae (ATCC 27956), Staphylococcus aureus (ATCC 29740), and Escherichia coli+ (a wild strain isolated from an Ohio cow 2956RR during the dry period) were used. Log reductions ranged from 5.6480 to 1.8330 for Strep. agalactiae compared with undipped controls. Staphylococcus aureus were reduced from 4.8972 to 1.0102. Escherichia coli were reduced from 5.8677 to 1.1450. The percentages of the products tested that had log reductions < 3 against Strep. agalactiae, Staph. aureus, and E. coli were 24, 30, and 61. Four teat dip formulations contained 4% sodium hypochlorite; all were effective against Strep. agalactiae, Staph. aureus, and E. coli. The three formulations containing glutaraldehyde were effective against the three mastitis pathogens. Formulations containing 1 and .5% iodine were not consistently effective against Strep. agalactiae, Staph. aureus, or E. coli. Teat sanitizers containing .5% chlorhexidine also showed inconsistency. Variations in formulation for products containing iodine or chlorhexidine affected germicidal activity.
采用离体乳头试验对57种乳头浸液配方的杀菌活性进行了测试。使用了无乳链球菌（ATCC 27956）、金黄色葡萄球菌（ATCC 29740）和大肠杆菌+（一种在干奶期从俄亥俄州一头奶牛2956RR分离出的野生菌株）。与未浸药的对照相比，无乳链球菌的对数减少量在5.6480至1.8330之间。金黄色葡萄球菌从4.8972减少到1.0102。大肠杆菌从5.8677减少到1.1450。对无乳链球菌、金黄色葡萄球菌和大肠杆菌的对数减少量<3的受试产品百分比分别为24%、30%和61%。四种乳头浸液配方含有4%的次氯酸钠；所有配方对无乳链球菌、金黄色葡萄球菌和大肠杆菌均有效。三种含有戊二醛的配方对三种乳腺炎病原体均有效。含有1%和0.5%碘的配方对无乳链球菌、金黄色葡萄球菌或大肠杆菌的效果并不一致。含有0.5%洗必泰的乳头消毒剂也表现出不一致性。含碘或洗必泰产品配方的变化会影响杀菌活性。
