Chen S, Beehler B, Sugimoto G, Tramposch K M
Bristol-Myers Squibb Pharmaceutical Research Institute, Buffalo, New York 14213.
J Invest Dermatol. 1993 Aug;101(2):237-9. doi: 10.1111/1523-1747.ep12365074.
All-trans retinoic acid (tRA) was previously shown to be active in wrinkle reduction in the hairless mouse photoaging model. To address the questions of whether tRA also alters glycosaminoglycan (GAG) synthesis and whether observed wrinkle effacement can be attributed to changes in total GAG production, the effects of tRA on de novo GAG synthesis were examined in this model. Sulfated glycosaminoglycans (S-GAG) and the non-sulfated hyaluronic acid (HA) labeled with [3H]-glucosamine or [35S]-sulfate were found to diffuse differentially into the medium during the labeling period in the control animals (9% and 35% of total incorporated label for HA and S-GAG, respectively). Furthermore, the diffusion of HA into media was significantly changed after tRA treatment (from 9% to 24%), but no alteration was observed in the diffusion of S-GAG. Separation of epidermis and dermis indicated that the additional HA in medium after retinoid treatment primarily originated from the dermis. When incorporated label from the medium and skin fractions was combined, both labeling protocols revealed that 10 weeks of tRA treatment did not increase the total (medium plus skin) de novo synthesis of either HA or S-GAG. Wrinkle effacement as induced by retinoids in the photodamaged mouse skin therefore can not be related to an increased total GAG synthesis.
全反式维甲酸（tRA）先前已被证明在无毛小鼠光老化模型中具有减少皱纹的活性。为了解决tRA是否也会改变糖胺聚糖（GAG）合成以及观察到的皱纹消退是否可归因于总GAG产生变化的问题，在该模型中研究了tRA对从头合成GAG的影响。在对照动物的标记期内，发现用[3H]-葡萄糖胺或[35S]-硫酸盐标记的硫酸化糖胺聚糖（S-GAG）和非硫酸化透明质酸（HA）向培养基中的扩散存在差异（HA和S-GAG分别占总掺入标记的9%和35%）。此外，tRA处理后HA向培养基中的扩散发生了显著变化（从9%变为24%），但未观察到S-GAG扩散的改变。表皮和真皮的分离表明，类维生素A处理后培养基中额外的HA主要来自真皮。当将培养基和皮肤部分的掺入标记结合起来时，两种标记方案均显示，10周的tRA处理并未增加HA或S-GAG的总（培养基加皮肤）从头合成。因此，类维生素A在光损伤小鼠皮肤中诱导的皱纹消退与总GAG合成增加无关。
