Wu X, Stewart S, Theilmann D A
Agriculture Canada Research Station, Vancouver, British Columbia.
J Gen Virol. 1993 Aug;74 ( Pt 8):1591-8. doi: 10.1099/0022-1317-74-8-1591.
A new gene of the baculovirus Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV) has been identified that encodes a highly basic 8.9K protein. The gene called p8.9 is expressed as a 0.5 kb transcript by 1 h post-infection and initiates at an early gene motif. The promoter of the 0.5 kb transcript has two upstream elements, repeats I and II, which are similar to motifs previously characterized in the OpMNPV IE-2 gene and the Autographa californica nuclear polyhedrosis virus IE-N and PE38 genes. A second p8.9 transcript expressed from 8 to 72 h post-infection was shown to initiate 634 bp upstream from the early gene motif in a region that has no similarity to any previously described baculovirus promoter or initiation site. Transient assays utilizing a reporter gene have shown that the p8.9 early promoter is active in a Lymantria dispar (LD652Y) and Spodoptera frugipeda (Sf9) cell lines in the absence of other viral factors. In addition, it was also demonstrated that the p8.9 promoter is trans-activated by the OpMNPV IE-2 and p34 genes, but not by IE-1.
已鉴定出杆状病毒伪黄杉毒蛾多粒包埋核型多角体病毒（OpMNPV）的一个新基因，其编码一种高度碱性的8.9K蛋白。这个名为p8.9的基因在感染后1小时以0.5 kb的转录本形式表达，并从一个早期基因基序开始。0.5 kb转录本的启动子有两个上游元件，重复序列I和II，它们与先前在OpMNPV IE - 2基因以及苜蓿银纹夜蛾核型多角体病毒IE - N和PE38基因中鉴定的基序相似。在感染后8至72小时表达的第二个p8.9转录本，显示在早期基因基序上游634 bp处起始，该区域与任何先前描述的杆状病毒启动子或起始位点均无相似性。利用报告基因进行的瞬时分析表明，在没有其他病毒因子的情况下，p8.9早期启动子在舞毒蛾（LD652Y）和草地贪夜蛾（Sf9）细胞系中具有活性。此外，还证明p8.9启动子可被OpMNPV IE - 2和p34基因反式激活，但不能被IE - 1激活。
