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A strong-stop DNA in rice plants infected with rice tungro bacilliform virus.

作者信息

Bao Y, Hull R

机构信息

Department of Virus Research, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, U.K.

出版信息

J Gen Virol. 1993 Aug;74 ( Pt 8):1611-6. doi: 10.1099/0022-1317-74-8-1611.

Abstract

A virus-specific small nucleic acid (strong-stop DNA) was identified in rice plants infected with rice tungro bacilliform virus, but not in the virus particles. This nucleic acid was shown to consist of about 595 deoxyribonucleotides with about 70 ribonucleotides covalently linked at the 5' end. Hybridization with sequence-specific oligonucleotides showed that the ribonucleotides were from the plant cytoplasmic tRNA(iMet) sequence. PCR analysis detected hairpin structures at the 3' end of the DNA.

摘要

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