Sawatani M, Yasueda H, Akiyama K, Shida T, Taniguchi Y, Usui M, Ando S, Kurimoto M, Matuhashi T
Hayashibara Biochemical Laboratories Inc.
Arerugi. 1993 Jun;42(6):738-47.
Cry j II, the second major allergen of Japanese cedar (sugi, Cryptomeria japonica) pollen was examined for the allergenicity by intradermal test and RAST. Nineteen of the 25 allergic patients examined, showed positive reaction to the Cry j II. Contents of Cry j II in the extracts of the pollen collected in various regions from 1977 to 1991 showed yearly variation ranging from 2.9 to 14 mg/100 g pollen, whereas the amount of Cry j I in the extract was comparatively stable at about 35 mg/100 g pollen. Physicochemical treatments of Cry j I and Cry j II suggested that specific human IgE antibodies and some mAbs bind to conformational epitopes which are denatured and destroyed by certain treatments.
通过皮内试验和放射变应原吸附试验（RAST）检测了日本柳杉花粉的第二大主要过敏原Cry j II的致敏性。在接受检测的25名过敏患者中，有19人对Cry j II呈阳性反应。1977年至1991年在不同地区采集的花粉提取物中Cry j II的含量显示出逐年变化，范围为2.9至14毫克/100克花粉，而提取物中Cry j I的含量相对稳定，约为35毫克/100克花粉。对Cry j I和Cry j II的物理化学处理表明，特定的人IgE抗体和一些单克隆抗体与构象表位结合，这些构象表位会被某些处理变性和破坏。
