de Bracco M M, Borda E, Galassi N, Perez-Bianco R, Sterin-Borda L
Career Investigators of Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), Argentina.
Autoimmunity. 1993;14(4):307-14. doi: 10.3109/08916939309079233.
In human immune deficiency virus (HIV) disease, direct infection of heart tissue with HIV and repeated intestinal infections with opportunistic pathogens are thought to be the main cause of cardiac disease and diarrhoea respectively. A role for autoimmune phenomena may also be involved in the pathogeny of HIV disease. In this study, we demonstrate that immunoglobulins from the A and G classes from HIV positive patients are able to interfere with the function of the muscarinic cholinergic receptors from heart and gut. Both IgA and IgG HIV+ preparations decreased the tension of isolated atria and increased the tension of isolated ileum. The mechanical effect of carbachol was inhibited in both atria and ileum preparations, when they were preincubated with either IgA or IgG HIV+ fractions. An inhibitor of muscarinic cholinergic receptors (atropine) impaired the negative inotropic action of HIV+ immunoglobulins (Ig) on the heart and prevented the positive inotropic effect of HIV+ Igs on ileum. HIV+ IgA fraction was approximately ten fold more potent to interfere with the cholinergic function as compared to the IgG fraction. These results suggest that antibodies present in HIV+ serum may also modulate muscle's cholinergic activity in the heart and ileum from HIV+patients.
在人类免疫缺陷病毒（HIV）疾病中，HIV直接感染心脏组织以及机会性病原体反复感染肠道分别被认为是心脏病和腹泻的主要原因。自身免疫现象在HIV疾病的发病机制中也可能起作用。在本研究中，我们证明HIV阳性患者的A类和G类免疫球蛋白能够干扰心脏和肠道毒蕈碱胆碱能受体的功能。HIV+的IgA和IgG制剂均降低了离体心房的张力，并增加了离体回肠的张力。当用HIV+的IgA或IgG组分预孵育时，卡巴胆碱对心房和回肠制剂的机械作用均受到抑制。毒蕈碱胆碱能受体抑制剂（阿托品）削弱了HIV+免疫球蛋白（Ig）对心脏的负性肌力作用，并阻止了HIV+ Igs对回肠的正性肌力作用。与IgG组分相比，HIV+的IgA组分干扰胆碱能功能的效力大约强十倍。这些结果表明，HIV+血清中的抗体也可能调节HIV+患者心脏和回肠中肌肉的胆碱能活性。
