Dell'Anna M E, Luthman J, Lindqvist E, Olson L
Experimental Neurology Laboratory-Institute of Neurology, Catholic University, Rome, Italy.
Brain Res Bull. 1993;32(2):159-70. doi: 10.1016/0361-9230(93)90070-r.
Neurochemical and morphological effects of neonatal anoxia on monoamine systems were studied after 100% N2 exposure for 25 min at 30 h postnatally (postnatal day 2-P2). At 20 min after anoxia, reductions of tissue levels of cerebellar noradrenaline (NA) and striatal dopamine (DA) and metabolites were seen, while 5-hydroxyindoleacetic acid (5-HIAA) was increased in cortex and cerebellum. At P7, NA increased in cerebellum, while serotonin (5-HT) and 5-HIAA decreased in cortex and cerebellum. At P21, increased hippocampal NA and striatal homovanillic acid (HVA) were found, while striatal 5-HT decreased and 5-HIAA increased in striatum and hippocampus. At P60, striatal 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-HIAA levels were found to be enhanced. No effects were seen on 5-HT, tyrosine hydroxylase, or DARPP-32 immunostaining in cortex, hippocampus, and striatum. Thus, the neonatal anoxia induced both acute and persistent neurochemical abnormalities in monoamine systems that were not accompanied by morphological changes detectable with the methods used. The monoamine alterations found could be critically connected to the behavioral disturbances observed in rats after neonatal anoxia. The findings may also be of relevance to dysfunctions seen in humans after perinatal oxygen deficiency, e.g., the attention deficit hyperactivity disorder syndrome.
研究了新生期缺氧对单胺系统的神经化学和形态学影响，方法是在出生后30小时（出生后第2天-P2）让幼鼠暴露于100%氮气中25分钟。缺氧后20分钟，可见小脑去甲肾上腺素（NA）、纹状体多巴胺（DA）及其代谢产物的组织水平降低，而皮质和小脑中5-羟吲哚乙酸（5-HIAA）增加。在P7时，小脑NA增加，而皮质和小脑中血清素（5-HT）和5-HIAA减少。在P21时，发现海马NA和纹状体高香草酸（HVA）增加，而纹状体5-HT减少，纹状体和海马中5-HIAA增加。在P60时，发现纹状体3,4-二羟基苯乙酸（DOPAC）和5-HIAA水平升高。在皮质、海马和纹状体中，5-HT、酪氨酸羟化酶或DARPP-32免疫染色未见影响。因此，新生期缺氧在单胺系统中诱导了急性和持续性神经化学异常，这些异常并未伴随所用方法可检测到的形态学变化。所发现的单胺改变可能与新生期缺氧后大鼠出现的行为障碍密切相关。这些发现也可能与围产期缺氧后人类出现的功能障碍有关，例如注意力缺陷多动障碍综合征。
