丝裂原刺激的淋巴细胞中腺苷脱氨酶的表面表达。
Surface expression of adenosine deaminase in mitogen-stimulated lymphocytes.
作者信息
Martin M, Centelles J J, Huguet J, Echevarne F, Colomer D, Vives-Corrons J L, Franco R
机构信息
Departament de Bioquímica i Fisiologia, Facultat de Química, Universitat de Barcelona, Spain.
出版信息
Clin Exp Immunol. 1993 Aug;93(2):286-91. doi: 10.1111/j.1365-2249.1993.tb07981.x.
Adenosine deaminase (ADA) expression on the surface of mitogen-stimulated lymphocytes was studied by flow cytometry. The gate for lymphocytes was located by cell size (forward scatter), cytoplasmic complexity (side scatter) and by expression of the markers CD2, CD4, CD8 and CD19. After mitogenic proliferation two populations appeared, one corresponding to non-stimulated cells, and the other consisting of larger cells which showed relatively high expression of adenosine deaminase on their surface. The increase was similar to that observed for CD71 expression, and paralleled the increase in 3H-thymidine incorporation. There was a correlation between ADA and CD71 expression (r = 0.92 for phytohaemagglutinin (PHA) and 0.97 for pokeweed mitogen (PWM)). These results suggest a role for ecto-adenosine deaminase in lymphocyte proliferation and/or triggering.
采用流式细胞术研究了丝裂原刺激的淋巴细胞表面腺苷脱氨酶（ADA）的表达。通过细胞大小（前向散射）、细胞质复杂性（侧向散射）以及标志物CD2、CD4、CD8和CD19的表达来确定淋巴细胞的门控。有丝分裂增殖后出现了两个群体，一个对应于未刺激的细胞，另一个由较大的细胞组成，这些细胞在其表面显示出相对较高的腺苷脱氨酶表达。这种增加与观察到的CD71表达的增加相似，并且与3H-胸苷掺入的增加平行。ADA与CD71表达之间存在相关性（植物血凝素（PHA）刺激时r = 0.92，商陆丝裂原（PWM）刺激时r = 0.97）。这些结果表明胞外腺苷脱氨酶在淋巴细胞增殖和/或触发中起作用。
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