Laboratory of Neurochemistry, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, MD 20014, U.S.A.
Biochem J. 1975 Dec;152(3):681-7. doi: 10.1042/bj1520681.
Two types of adenosine deaminase (EC 3.5.4.4) were found in cultured cells of central-nervous-system origin. The predominant and more active enzyme was obtained in soluble form from the cytosol of mouse neuroblastoma (N-18), neonatal hamster astrocytes (NN), human oligodendroglioma (HOL) and human astrocytoma (Cox Clone). Particulate adenosine deaminase was probably associated with the plasma membrane. When radioactive adenosine was added to superfusates of monolayer cultures it was rapidly converted into inosine and hypoxanthine. The metabolic conversion required adenosine uptake by the cells, a probable transition through the intracellular ATP pool(s) and a rapid excretion into the superfusate of the catabolic products. We discuss the evidence that points to adenosine and its derivatives as neurohumoral modulators of central-nervous-system function.
两种类型的腺苷脱氨酶（EC3.5.4.4）存在于中枢神经系统来源的培养细胞中。从鼠神经母细胞瘤（N-18）、新生仓鼠星形胶质细胞（NN）、人少突胶质细胞瘤（HOL）和人星形细胞瘤（Cox Clone）的细胞质中可获得主要的、更活跃的可溶性酶。颗粒腺苷脱氨酶可能与质膜相关。当放射性腺苷被添加到单层培养物的灌流液中时，它会迅速转化为肌苷和次黄嘌呤。这种代谢转化需要细胞摄取腺苷，可能通过细胞内 ATP 池（多个）进行转化，并迅速将代谢产物排泄到灌流液中。我们讨论了以下证据：指出腺苷及其衍生物是中枢神经系统功能的神经调质。
