牛富含组氨酸糖蛋白中二硫键桥排列的测定。

Determination of the disulphide bridge arrangement of bovine histidine-rich glycoprotein.

作者信息

Sørensen C B, Krogh-Pedersen H, Petersen T E

机构信息

Department of Molecular Biology, University of Aarhus, Denmark.

出版信息

FEBS Lett. 1993 Aug 16;328(3):285-90. doi: 10.1016/0014-5793(93)80945-q.

DOI:10.1016/0014-5793(93)80945-q

PMID:8348977

Abstract

Histidine-rich glycoprotein (HRG) was purified from bovine plasma and the disulphide bridge arrangement established. Disulphide-bridged peptides were obtained from peptic and tryptic degradation of native bovine HRG. Twelve half-cystine residues were found in bovine HRG (compared to sixteen cysteines in human HRG), all involved in the formation of six disulphide bridges connecting Cys-1 to Cys-12, Cys-2 to Cys-3, Cys-4 to Cys-5, Cys-6 to Cys-11, Cys-7 to Cys-8, and Cys-9 to Cys-10. Additional sequence analysis of 14C-carboxymethylated chymotryptic and Staphylococcus aureus V8 protease generated peptides and CNBr-fragments of bovine HRG yielded a partial amino acid sequence of bovine HRG constituting 78% of the sequence when compared to the human cDNA sequence.

摘要

从牛血浆中纯化出富含组氨酸的糖蛋白（HRG），并确定了其二硫键排列方式。通过对天然牛HRG进行胃蛋白酶和胰蛋白酶降解获得了二硫键连接的肽段。在牛HRG中发现了12个半胱氨酸残基（相比之下，人HRG中有16个半胱氨酸），所有这些残基都参与形成6个二硫键，分别连接Cys-1与Cys-12、Cys-2与Cys-3、Cys-4与Cys-5、Cys-6与Cys-11、Cys-7与Cys-8以及Cys-9与Cys-10。对经14C-羧甲基化的胰凝乳蛋白酶和金黄色葡萄球菌V8蛋白酶产生的肽段以及牛HRG的溴化氰片段进行的额外序列分析，得到了牛HRG的部分氨基酸序列，与人类cDNA序列相比，该序列占总序列的78%。