Teruya M, Takei S, Forrest L E, Grunewald A, Chan E K, Charles M A
Department of Medicine, University of California, Irvine 92717.
Diabetes. 1993 Sep;42(9):1310-7. doi: 10.2337/diab.42.9.1310.
A decreased acute insulin response to glucose in islet cell antibody positive humans predicts diabetes. Because the dominant mechanism leading to decreased in vivo acute insulin response to glucose remains unclear, perifused islets were examined before and after diabetes onset in BB rats to assess the role of glucose sensitivity on insulin secretion in individual islets. Islets from normal WF rats, diabetes-prone rats without inflamed islets, diabetes-prone rats with inflamed islets, and diabetic rats were studied at 2.0, 8.3, and 16.7 mM glucose. Immunoreactive insulin from WF islets at 16.7 mM glucose was 0.15 +/- 0.02 ng.0-7 min-1 x islet-1 for the first phase and 1.00 +/- 0.05 ng.7-20 min-1 x islet-1 for the second phase of biphasic secretion, compared with basal secretion of 0.10 +/- 0.03 ng.20 min-1 x islet-1 at 2 mM glucose. Diabetes-prone noninflamed islets showed a 0.20 +/- 0.03 ng first-phase secretion, a 1.32 +/- 0.13 ng second-phase secretion after 16.7 mM glucose, and 0.093 +/- 0.02 ng.20 min-1 x islet-1 at 2 mM glucose, indicating no intrinsic BB rat strain secretion abnormality. Diabetes-prone inflamed islets had secretions of 0.35 +/- 0.02 ng during the first phase (P < 0.05 vs. WF) and 1.78 +/- 0.29 ng during the second phase (P < 0.05 vs. WF) after 16.7 mM glucose, with 0.24 +/- 0.08 ng.20 min-1 x islet-1 at 2 mM glucose.(ABSTRACT TRUNCATED AT 250 WORDS)
胰岛细胞抗体阳性人群对葡萄糖的急性胰岛素反应降低预示着糖尿病。由于导致体内对葡萄糖的急性胰岛素反应降低的主要机制尚不清楚，因此在BB大鼠糖尿病发病前后对分离灌注的胰岛进行检查，以评估葡萄糖敏感性对单个胰岛胰岛素分泌的作用。在2.0、8.3和16.7 mM葡萄糖浓度下，对正常WF大鼠、无胰岛炎症的糖尿病倾向大鼠、有胰岛炎症的糖尿病倾向大鼠以及糖尿病大鼠的胰岛进行了研究。WF胰岛在16.7 mM葡萄糖浓度下，双相分泌的第一相免疫反应性胰岛素为0.15±0.02 ng·0 - 7 min - 1·胰岛 - 1，第二相为1.00±0.05 ng·7 - 20 min - 1·胰岛 - 1，而在2 mM葡萄糖浓度下基础分泌为0.10±0.03 ng·20 min - 1·胰岛 - 1。无胰岛炎症的糖尿病倾向胰岛在16.7 mM葡萄糖浓度下第一相分泌为0.20±0.03 ng，第二相分泌为1.32±0.13 ng，在2 mM葡萄糖浓度下为0.093±0.02 ng·20 min - 1·胰岛 - 1，表明BB大鼠品系本身无分泌异常。有胰岛炎症的糖尿病倾向胰岛在16.7 mM葡萄糖浓度下第一相分泌为0.35±0.02 ng（与WF相比P < 0.05），第二相分泌为1.78±0.29 ng（与WF相比P < 0.05），在2 mM葡萄糖浓度下为0.24±0.08 ng·20 min - 1·胰岛 - 1。（摘要截选至250字）
