Provencher D M, Finstad C L, Saigo P E, Rubin S C, Hoskins W J, Federici M G, Stockert E, Lloyd K O, Lewis J L
Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Gynecol Oncol. 1993 Jul;50(1):78-83. doi: 10.1006/gyno.1993.1167.
The antigenic phenotype of malignant cells from ascites of patients with epithelial ovarian cancers was examined and compared to that of their primary and metastatic sites. Cell-surface antigens on frozen sections of primary and metastatic tumors and frozen cell pellets from ascites were analyzed with a panel of murine monoclonal antibodies using the indirect immunoperoxidase method. In addition, ascites cells cultured with and without autologous cell-free ascitic fluid were evaluated by immunofluorescence. The pattern of antigen expression detected on fresh and cultured ascitic epithelial cells was shown to be identical to the expression in autologous solid tumor tissues. When placed in culture, malignant epithelial cells generally persisted for a minimum of one, but no more than five, passages. Addition of autologous ascitic fluid to cultures of ascites cells did not alter the phenotype of the epithelial tumor cell population and did not enhance the growth of these cells. From one culture of ascites cells a permanent malignant epithelial ovarian cancer cell line (designated SK-OV-8) was established. The demonstration that epithelial tumor cells found in ascites of patients with epithelial ovarian cancer have the identical antigenic phenotype as their solid tumor counterpart, at least for the panel of antigens studied, may be useful in planning imaging and therapeutic trials with monoclonal antibodies.
对上皮性卵巢癌患者腹水中恶性细胞的抗原表型进行了检测，并与它们的原发部位和转移部位的抗原表型进行了比较。使用间接免疫过氧化物酶法，用一组鼠单克隆抗体分析原发性和转移性肿瘤冰冻切片以及腹水中冰冻细胞沉淀上的细胞表面抗原。此外，通过免疫荧光对在有和没有自体无细胞腹水的情况下培养的腹水细胞进行了评估。在新鲜和培养的腹水上皮细胞上检测到的抗原表达模式与自体实体瘤组织中的表达相同。当置于培养中时，恶性上皮细胞通常至少能传代一次，但不超过五次。向腹水细胞培养物中添加自体腹水不会改变上皮肿瘤细胞群体的表型，也不会促进这些细胞的生长。从一种腹水细胞培养物中建立了一个永久性的恶性上皮性卵巢癌细胞系（命名为SK-OV-8）。上皮性卵巢癌患者腹水中发现的上皮肿瘤细胞与其实体瘤对应物具有相同的抗原表型，至少对于所研究的一组抗原来说是如此，这一发现可能有助于规划使用单克隆抗体的成像和治疗试验。
