Takai S, Watanabe Y, Ikeda T, Ozawa T, Matsukura S, Tamada Y, Tsubaki S, Sekizaki T
Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
J Clin Microbiol. 1993 Jul;31(7):1726-9. doi: 10.1128/jcm.31.7.1726-1729.1993.
Twenty-three strains of Rhodococcus equi from independent clinical cases were analyzed for the presence of virulence plasmid DNA. Of the clinical isolates, 19 contained an 85-kb plasmid and the remaining 4 contained a 90-kb plasmid. All of the isolates expressed 15- to 17-kDa antigens and were virulent in mice. Restriction enzyme and Southern blot analyses showed large regions of DNA homology between the 85- and 90-kb virulence plasmids. It was concluded tentatively that there are at least two virulence plasmids in R. equi and that they have a common origin.
对来自独立临床病例的23株马红球菌进行了毒力质粒DNA检测分析。在这些临床分离株中，19株含有一个85kb的质粒，其余4株含有一个90kb的质粒。所有分离株均表达15至17kDa的抗原，且对小鼠具有毒力。限制性内切酶分析和Southern印迹分析表明，85kb和90kb的毒力质粒之间存在大片段DNA同源性。初步得出结论，马红球菌中至少存在两种毒力质粒，且它们有共同的起源。
