[泥鳅卵细胞的脱氧核糖核酸酶和核糖核酸酶]

[DNases and RNases of Misgurnus fossilis ovocytes].

作者信息

Kusen' S J, Oleshko P S

出版信息

Ukr Biokhim Zh. 1975 Nov-Dec;47(6):719-23.

PMID:835

Abstract

The pH optima were determined for DNases and RNases of the loach eggs. For DNases they are 5.6 and 7.6 and for RNases - 5.2 and 7.2. It is established that Ca++ activates, and Fe++ has not effect on the activity of acid and alkaline DNases, while Mg++, Mn++ and especially Co++, Zn++, Cd++, Cu++ have an inhibitory effect on them. The activities of RNases is stimulated by Ca++ and Fe++, and inhibited by Zn++, Co++, Cd++ and Cu++. Iones Mg++ and Mn++ do not affect these activities. Localization of the above mentioned enzymes was studied by means of differential centrifugation of egg homogenates. Acid DNase is concentrated only in postmicrosomal supernatant liquid, its activity being inhibited in the presence of the nucleomitochondrial and microsomal fractions. Acid RNase is also localized predominantly in postmicrosomal supernatant fraction. Alkaline DNase is found to a great extent in nucleomitochondrial fraction, and alkaline RNase - in postmicrosomal one.

摘要

测定了泥鳅卵中脱氧核糖核酸酶（DNase）和核糖核酸酶（RNase）的最适pH值。DNase的最适pH值为5.6和7.6，RNase的最适pH值为5.2和7.2。已确定Ca++能激活酸性和碱性DNase的活性，而Fe++对其活性无影响，而Mg++、Mn++，尤其是Co++、Zn++、Cd++、Cu++对其有抑制作用。RNase的活性受Ca++和Fe++刺激，而受Zn++、Co++、Cd++和Cu++抑制。Mg++和Mn++离子对这些活性无影响。通过对卵匀浆进行差速离心研究了上述酶的定位。酸性DNase仅集中在微粒体后上清液中，其活性在核线粒体和微粒体部分存在时受到抑制。酸性RNase也主要定位于微粒体后上清部分。碱性DNase在很大程度上存在于核线粒体部分，碱性RNase则存在于微粒体后部分。