一种与酵母中Ras负调控因子相关的视网膜母细胞瘤结合蛋白。

A retinoblastoma-binding protein related to a negative regulator of Ras in yeast.

作者信息

Qian Y W, Wang Y C, Hollingsworth R E, Jones D, Ling N, Lee E Y

机构信息

Center for Molecular Medicine, University of Texas, Health Science Center, San Antonio 78245-3207.

出版信息

Nature. 1993 Aug 12;364(6438):648-52. doi: 10.1038/364648a0.

DOI:10.1038/364648a0

PMID:8350924

Abstract

The growth suppression function of the retinoblastoma protein (Rb) is though to be mediated by Rb binding to cellular proteins. p48 is one of the major proteins that binds to a putative functional domain at the carboxy terminus of the Rb protein. Here we report the isolation of a full-length complementary DNA (RbAp48) encoding p48. Complex formation between p48 and Rb occurs in vitro and in vivo, and apparently involves direct interaction between the proteins. Like Rb, p48 is a ubiquitously expressed nuclear protein. RbAp48 share sequence homology with MSI1, a negative regulator of the Ras-cyclic AMP pathway in the yeast Saccharomyces cerevisiae. Furthermore, like MSI1, human RbAp48 suppresses the heat-shock sensitivity of the yeast ira1 strains and RAS2Val19 strains. Interaction with p48 may be one of the mechanisms for suppression of growth mediated by Rb.

摘要

视网膜母细胞瘤蛋白（Rb）的生长抑制功能被认为是由Rb与细胞蛋白结合介导的。p48是与Rb蛋白羧基末端一个假定功能域结合的主要蛋白之一。在此，我们报告了编码p48的全长互补DNA（RbAp48）的分离。p48与Rb之间的复合物形成在体外和体内均会发生，且显然涉及蛋白质之间的直接相互作用。与Rb一样，p48是一种广泛表达的核蛋白。RbAp48与酿酒酵母中Ras-环磷酸腺苷途径的负调节因子MSI1具有序列同源性。此外，与MSI1一样，人RbAp48可抑制酵母ira1菌株和RAS2Val19菌株的热休克敏感性。与p48相互作用可能是Rb介导生长抑制的机制之一。