细胞因子、视黄酸和1,25(OH)₂-维生素D₃对人造血细胞系细胞表面白细胞唾液酸蛋白（涎福林、CD43抗原）的调节作用。

Modulation of leukosialin (sialophorin, CD43 antigen) on the cell surface of human hematopoietic cell lines induced by cytokins, retinoic acid and 1,25(OH)2-vitamin D3.

作者信息

Turzová M, Hunáková L, Duraj J, Speiser P, Sedlák J, Chorváth B

机构信息

Cancer Research Institute, Slovak Academy of Sciences, Bratislava.

出版信息

Neoplasma. 1993;40(1):9-13.

PMID:8350952

Abstract

Cell surface expression of leukosialin (sialophorin, CD43 antigen) on human neoplastic hematopoietic cell lines K-562, U-937, HL-60 and REH was determined with the aid of a new CD43 monoclonal antibody (Bra7G) by the immunochemical (radioimmunoprecipitation, immunoblotting) and immunocytofluorometric techniques. Interferon-gamma and TNF-alpha were utilized as the "physiological" inducers of differentiation-associated markers. The "non-physiological" inducer phorbol ester PMA induced down-regulation of leukosialin cell surface expression on immature erythroid-myeloid leukemia cell line K-562, but up-regulation of CD43 antigen on the promyelocyte leukemia cell line HL-60 and, to a lesser extent on the monocyte-like U-937 and CALLA+ ALL cell line REH. Retinoic acid down-regulated leukosialin on both U-937 monocyte-like cells and the CALLA+ ALL cell line REH. In contrast to these data, interferon-gamma, TNF-alpha, retinoic acid and 1,25(OH)2-vitamin D3 induced the up-regulation of leukosialin in a promyelocytic leukemia cell line HL-60.

摘要

借助新型CD43单克隆抗体（Bra7G），通过免疫化学（放射免疫沉淀、免疫印迹）和免疫细胞荧光技术，测定了人肿瘤造血细胞系K-562、U-937、HL-60和REH上白细胞唾液酸蛋白（涎福林、CD43抗原）的细胞表面表达。γ干扰素和肿瘤坏死因子-α被用作分化相关标志物的“生理性”诱导剂。“非生理性”诱导剂佛波酯PMA可诱导未成熟红系-髓系白血病细胞系K-562上白细胞唾液酸蛋白细胞表面表达下调，但可诱导早幼粒细胞白血病细胞系HL-60上CD43抗原表达上调，在单核细胞样U-937和CALLA+急性淋巴细胞白血病细胞系REH上诱导程度较轻。维甲酸可下调U-937单核细胞样细胞和CALLA+急性淋巴细胞白血病细胞系REH上的白细胞唾液酸蛋白。与这些数据相反，γ干扰素、肿瘤坏死因子-α、维甲酸和1,25(OH)2-维生素D3可诱导早幼粒细胞白血病细胞系HL-60上白细胞唾液酸蛋白表达上调。