Parker D B, McRory J E, Fischer W H, Park M, Sherwood N M
Biology Department, University of Victoria, Canada.
Regul Pept. 1993 Jun 11;45(3):379-86. doi: 10.1016/0167-0115(93)90364-e.
The purification of NPY from brains of the American alligator (Alligator mississippiensis) was achieved using reverse-phase high performance liquid chromatography (HPLC). The amino acid sequence was determined using automated Edman degradation as Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu- Asp-Ala-Pro-Ala-Glu-Asp-Met-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile- Asn-Leu - Ile-Thr-Arg-Gln-Arg-Tyr. Alligator NPY is the first non-mammalian vertebrate to have 100% sequence identity to human NPY. The conservation of alligator NPY suggests that serine in position 7 of chicken NPY evolved after the birds and reptiles diverged from a common Archosaurian ancestor. Furthermore, the sequence identity between alligator and human NPY suggests this sequence is the same as the ancestral amniote NPY.
利用反相高效液相色谱法（HPLC）从美国短吻鳄（密西西比鳄）的大脑中纯化出神经肽Y（NPY）。使用自动埃德曼降解法测定其氨基酸序列为Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Met-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr。短吻鳄NPY是首个与人类NPY具有100%序列同一性的非哺乳类脊椎动物。短吻鳄NPY的保守性表明，鸡NPY第7位的丝氨酸是在鸟类和爬行动物从共同的祖龙祖先分化之后进化而来的。此外，短吻鳄和人类NPY之间的序列同一性表明该序列与原始羊膜动物NPY相同。
