虹鳟（Oncorhynchus mykiss）神经肽Y。

Rainbow trout (Oncorhynchus mykiss) neuropeptide Y.

作者信息

Barton C L, Shaw C, Halton D W, Thim L

机构信息

School of Biology, Queen's University of Belfast, Northern Ireland.

出版信息

Peptides. 1992 Nov-Dec;13(6):1159-63. doi: 10.1016/0196-9781(92)90023-v.

DOI:10.1016/0196-9781(92)90023-v

PMID:1494498

Abstract

Neuropeptide Y (NPY) has been isolated from brain extracts of the rainbow trout (Oncorhynchus mykiss) and subjected to structural analyses. Plasma desorption mass spectroscopy estimated the molecular mass of the purified peptide as 4303.9 Da. Automated Edman degradation unequivocally established the sequence of a 36 amino acid residue peptide as: Tyr-Pro-Pro-Lys-Pro-Glu-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Pro-Glu-Glu-Leu-Ala- Lys- Tyr-Tyr-Thr-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr. The molecular mass calculated from this sequence (4304 Da) is consistent with that obtained by mass spectroscopy. The presence of a C-terminal amide was established by radioimmunoassay. Rainbow trout NPY is identical in primary structure to coho salmon (Oncorhynchus kisutch) pancreatic polypeptide (PP). These data may indicate that, in this group of salmonid fishes, a single member of the NPY/PP peptide family is expressed in both neurons and peripheral endocrine cells.

摘要

神经肽Y（NPY）已从虹鳟鱼（Oncorhynchus mykiss）的脑提取物中分离出来，并进行了结构分析。等离子体解吸质谱法估计纯化肽的分子量为4303.9道尔顿。自动Edman降解明确确定了一个36个氨基酸残基肽的序列为：Tyr-Pro-Pro-Lys-Pro-Glu-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Pro-Glu-Glu-Leu-Ala-Lys-Tyr-Tyr-Thr-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr。根据该序列计算出的分子量（4304道尔顿）与通过质谱法获得的分子量一致。通过放射免疫测定法确定了C末端酰胺的存在。虹鳟鱼NPY在一级结构上与银大麻哈鱼（Oncorhynchus kisutch）的胰多肽（PP）相同。这些数据可能表明，在这组鲑科鱼类中，NPY/PP肽家族的单个成员在神经元和外周内分泌细胞中均有表达。