[电磁场对枯草芽孢杆菌316 m来源的枯草杆菌蛋白酶的影响]
[Effect of an electromagnetic field on subtilisin from Bacillus subtilis 316 m].
作者信息
Povaliaeva I V, Koltukova N V, Kudria V A
出版信息
Ukr Biokhim Zh (1978). 1993 Jan-Feb;65(1):104-6.
PMID:8351733
Abstract
The influence of electromagnetic field on the subtilisin of Bacillus subtilis strain 316 M preparation has been studied. It is the increase 30-60% of the proteolytic activity during 60-100 min treatment was observed. The effect of activation is stable for a month at +4 degrees C. The increase of the proteolytic activity is connected with conformational changes of the enzyme molecule, with the decrease of pI from 11.4 to 9.2 in particular.
摘要
研究了电磁场对枯草芽孢杆菌316M菌株制备的枯草杆菌蛋白酶的影响。观察到在60 - 100分钟的处理过程中，蛋白水解活性提高了30 - 60%。在+4℃下，激活作用的效果可稳定一个月。蛋白水解活性的提高与酶分子的构象变化有关，特别是pI从11.4降至9.2。
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