精胺对豚鼠精子体外获能过程中钙内流的抑制作用。

Inhibition of spermine on calcium influx during capacitation of guinea pig spermatozoa in vitro.

作者信息

Zhong C L, Xin X H, Shi Q X

机构信息

Zhejiang Academy of Medical Sciences, Hangzhou, China.

出版信息

Zhongguo Yao Li Xue Bao. 1993 Mar;14(2):141-4.

PMID:8352007

Abstract

To investigate the mechanism of action of spermine, we measured the intracellular calcium ([Ca]i) of guinea pig spermatozoa using a probe of fluorescence, Quin 2. Results showed that spermine (0.25-2.0 mmol.L-1) suppressed the membrane permeability to Ca2+ during capacitation, which was similar to that of verapamil (a Ca2+ channel blocker). Furthermore, the rapid increase of [Ca]i induced by calcimycin (A-23187) was inhibited by spermine and verapamil, whereas trifluoperazine (an inhibitor of calmodulin) had no effect on it. The inhibition of the acrosome reaction caused by verapamil (5-100 mumol.L-1) or trifluoperazine (1-60 mumol.L-1) was reversed by calcimycin and cAMP, respectively. In addition, there was no effect on the initiation of the acrosome reaction when verapamil was added to capacitated spermatozoa. This result was in agreement with that of spermine. When addition of spermine (0.5 mmol.L-1) was combined with trifluoperazine (5 mumol.L-1), the acrosome reaction decline almost to zero, indicating that spermine might inhibit Ca2+ sensitive channel during sperm capacitation.

摘要

为了研究精胺的作用机制，我们使用荧光探针喹啉 2 测量了豚鼠精子的细胞内钙（[Ca]i）。结果表明，精胺（0.25 - 2.0 mmol·L-1）在获能过程中抑制了精子膜对 Ca2+ 的通透性，这与维拉帕米（一种 Ca2+ 通道阻滞剂）的作用相似。此外，离子霉素（A - 23187）诱导的 [Ca]i 的快速增加受到精胺和维拉帕米的抑制，而三氟拉嗪（一种钙调蛋白抑制剂）对此没有影响。维拉帕米（5 - 100 μmol·L-1）或三氟拉嗪（1 - 60 μmol·L-1）引起的顶体反应抑制分别被离子霉素和 cAMP 逆转。此外，当将维拉帕米添加到已获能的精子中时，对顶体反应的起始没有影响。这一结果与精胺的结果一致。当添加精胺（0.5 mmol·L-1）与三氟拉嗪（5 μmol·L-1）联合使用时，顶体反应下降几乎为零，表明精胺可能在精子获能过程中抑制 Ca2+ 敏感通道。